A simple, novel, accurate, precise, linear, rapid and economical RP-HPLC method was developed for the estimation of abacavir. The chromatographic separation was achieved using a Grace Smart RP 18 (250 x 4.6 mm, 5 �¼) column and isocratic elution, a mobile phase comprising of Methanol: 0.005 mM 1-Octanesulphonic acid at pH 5.0 was adjusted with O-Phosphoric acid in the ratio of 60:40 (v/v). The flow rate was 1.0 ml/min with detection at 280 nm using a UV detector and drug eluted with retention time of 4.157 min. The calibration curves were linear (r�²=0.9994) in the concentration range of 2-10 �¼g/ml. The limit of detection and limit of quantitation were found to be 0.6070 and 1.821 �¼g/ml respectively. Thus the simple, novel, economical, accurate, precise and rapid RP-HPLC method was developed for estimation of abacavir and validated as per ICH guidelines. Hence the proposed method was successfully applied for the quantitative estimation of abacavir in pure and pharmaceutical dosage form.
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